RNA Preparation and Purification
New England Biolabs, Inc. NEBNext Cell Lysis Buffer Module – 96 reactions
This module contains the enzymes and buffers required to lyse isolated cultured and primary cells to extract RNA. The module is optimized for use with the NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina or NEBNext Single Cell/Low Input cDNA Synthesis and Amplification Module, and can be used to supplement the NEBNext Cell Lysis Buffer and Murine RNase Inhibitor components supplied in these products.
New England Biolabs, Inc. Monarch® Spin Columns S2A and Tubes - 100 preps
The Monarch Spin Columns S2A and Tubes are a component of Monarch kits for RNA cleanup, and also offered separately for convenience and flexibility. The columns are designed without the use of a retaining ring, helping to ensure no buffer retention and no carryover of contaminants.
- Monarch spin columns and collection tubes designed for nucleic acid purification
- Minimal to no buffer retention/risk of carry-over contamination
- Low elution volume from unique column design
- Included in Monarch kits, also offered separately for flexibility
- Made with less plastic than conventional columns from leading suppliers
New England Biolabs, Inc. NEBNext Globin & rRNA Depletion Kit (Human/Mouse/Rat) with Sample Purification Beads – 96 reactions
The NEBNext Globin & rRNA Depletion Kit (Human/Mouse/Rat) employs the NEBNext RNase H-based RNA depletion workflow to deplete adult, embryonic and fetal globin mRNA (HBA1/2, HBB, HBD, HBM, HBG1/2, HBE1, HBQ1 and HBZ), cytoplasmic rRNA (5S, 5.8S, 18S, 28S, ITS and ETS) and mitochondrial rRNA (12S and 16S). The kit is effective with human, mouse and rat total RNA preparations, both intact and degraded. The resulting depleted RNA is suitable for RNA-seq, random-primed cDNA synthesis, or other downstream RNA analysis. This kit can also be used following poly(A) mRNA enrichment (e.g., using the NEBNext poly(A) mRNA Magnetic Isolation Module NEB #E7490), so that the final depleted RNA contains only mRNA of interest and no non-coding RNA. For convenience, this kit includes RNA Clean beads.
Norgen Biotek Corp Total RNA Purification 96-Well Kit
This 96-well kit provides a rapid method for the high-throughput isolation and purification of total RNA in 30 minutes using vacuum manifold, plate centrifuge or liquid handlers with vacuum capabilities. Total RNA can be isolated from a broad range of sample sources including cultured cells, tissues, blood, serum, plasma, bacteria, yeast, fungi, and viruses. The eluted RNA is consistent from well-to-well with high quality and purity, with excellent RIN values and A260/A280, and is suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS and more. The kit purifies all sizes of RNA from large mRNA, lncRNA down to microRNA (miRNA) in the same fraction without the requirement of phenol. Isolate all RNA sequences at an equal rate irrespective of size. Moreover, when the RNA sequences are small (e.g. miRNA), the column binds small RNAs regardless of their GC content.
Omega Biotek Inc Mag-Bind Total RNA 96 Kit | 4 x 96 preps
The Mag-Bind Total RNA 96 Kit provides a novel technology for total RNA isolation. This kit allows the rapid and reliable isolation of high-quality total cellular RNA and viral RNA from a wide variety of cells and tissues. Unlike column-based systems, the binding of nucleic acids to magnetic particles occurs in solution resulting in increased binding kinetics and binding efficiency. Particles are also completely re-suspended during the wash steps of the purification protocol, which improves the removal of contaminants and increases nucleic acid purity. Mag-Bind Total RNA 96 Kit procedure can be fully automated with most robotic workstations.
Research Products International Corp Direct-zol RNA MiniPrep w/Zymo-Spin IIC Columns, Capped, 50 Preps
The Direct-zol RNA MiniPrep Kits are RNA purification kits that provide a streamlined method for the purification of up to 50 µg (per prep) of high-quality RNA directly from samples in TRI Reagent or similar. Total RNA, including small RNAs (17-200 nt), is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, biological liquids, etc.). Isolation of RNA by conventional phase separation was shown to selectively enrich for some species of miRNA, leading to bias in downstream analysis. The Direct-zol method assures unbiased recovery of small RNAs including miRNA. The procedure is easy. Simply apply a prepared sample in TRI Reagent® directly to the Zymo-Spin Column and then bind, wash, and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary.
Research Products International Corp Direct-zol RNA MiniPrep Plus w/TRI Reagent, & Zymo-Spin IIICG Columns, Capped, 50 Preps
The Direct-zol RNA MiniPrep Plus Kits are RNA purification kits that provide a streamlined method for the purification of up to 100 µg (per prep) of high-quality RNA directly from samples in TRI Reagent® or similar. Total RNA, including small RNAs (17-200 nt), is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, biological liquids, etc.). Isolation of RNA by conventional phase separation was shown to selectively enrich for some species of miRNA, leading to bias in downstream analysis. The Direct-zol method assures unbiased recovery of small RNAs including miRNA. The procedure is easy. Simply apply a prepared sample in TRI Reagent® directly to the Zymo-Spin Column and then bind, wash, and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary.
Research Products International Corp Direct-zol RNA MiniPrep w/TRI Reagent & Zymo-Spin IIC Columns, Capped, 50 Preps
The Direct-zol RNA MiniPrep Kits are RNA purification kits that provide a streamlined method for the purification of up to 50 µg (per prep) of high-quality RNA directly from samples in TRI Reagent or similar. Total RNA, including small RNAs (17-200 nt), is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, biological liquids, etc.). Isolation of RNA by conventional phase separation was shown to selectively enrich for some species of miRNA, leading to bias in downstream analysis. The Direct-zol method assures unbiased recovery of small RNAs including miRNA. The procedure is easy. Simply apply a prepared sample in TRI Reagent® directly to the Zymo-Spin Column and then bind, wash, and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary.
Research Products International Corp Direct-zol RNA MiniPrep w/Zymo-Spin IIC Columns, Capped, 200 Preps
The Direct-zol RNA MiniPrep Kits are RNA purification kits that provide a streamlined method for the purification of up to 50 µg (per prep) of high-quality RNA directly from samples in TRI Reagent or similar. Total RNA, including small RNAs (17-200 nt), is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, biological liquids, etc.). Isolation of RNA by conventional phase separation was shown to selectively enrich for some species of miRNA, leading to bias in downstream analysis. The Direct-zol method assures unbiased recovery of small RNAs including miRNA. The procedure is easy. Simply apply a prepared sample in TRI Reagent® directly to the Zymo-Spin Column and then bind, wash, and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary.
Zymo Research Corporation Direct-zol™ RNA MiniPrep (200 Preps) w/ Zymo-Spin™ IIC Columns (Capped)
Total RNA including small RNAs (17-200 nt)2, is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, samples stored in DNA/RNA Shield™, etc.).Simply add ethanol to a TRI Reagent sample, bind directly to the Zymo-Spin™ Column, wash, and elute RNA. No phase separation, precipitation, or post-purification steps are necessary. RNA is high-quality and ready for Next-Gen Sequencing, RTqPCR, transcription profiling, hybridization, etc.
Zymo Research Corporation Direct-zol-96 RNA (2x96 Preps)
by using this product, total RNA, including small RNAs (17-200 nt), is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, biological liquids, etc.)The extraction method inactivates viruses and other infectious agents.The procedure is easy: simply apply a sample in TRI Reagent to the Zymo-Spin™ I-96 Plate, then bind, wash, and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary. The result is high-quality RNA suitable for subsequent RNA-based methods including Next-Gen sequencing, RT-qPCR, hybridization, etc.
Beckman Coulter FormaPure XL RNA Reagent Kit, 50 Preps
RNA extraction from FFPE samples have been demonstrated using this kit. This method can be performed in 1.5 mL tube format or in a 96-well 1.2 mL plate format. There are enough materials in this kit for 50 preps.